Citation

Please cite this package if it is used in any publication. The citation details can be accessed in the command line:

citation("AquaticLifeHistory")
#> To cite AquaticLifeHistory use:
#> 
#>   Smart J 2023 AquaticLifeHistory: Fisheries life history analysis
#>   using contemporary methods.
#>   https://github.com/jonathansmart/AquaticLifeHistory
#> 
#>   Smart, J. J., Chin, A. , Tobin, A. J. and Simpfendorfer, C. A. (2016)
#>   Multimodel approaches in shark and ray growth studies: strengths,
#>   weaknesses and the future. Fish and Fisheries, 17: 955-971.
#>   doi:10.1111/faf.12154
#> 
#> To see these entries in BibTeX format, use 'print(<citation>,
#> bibtex=TRUE)', 'toBibtex(.)', or set
#> 'options(citation.bibtex.max=999)'.

Introduction

Growth information attained through length-at-age analysis is a key component in many fisheries analyses. This package gives the user the ability to quickly and efficiently estimate growth for an aquatic species (fishes, sharks, molluscs, crustaceans, etc.) using robust and contemporary techniques. This can be achieved using either a single model approach (i.e. application of a von Bertalanffy growth function) or through a multi-model approach where multiple models are applied simultaneously and compared.This package will provide parameter estimates with errors, length-at-age estimates with bootstrapped confidence intervals, plots and model statistics - providing users with everything required for scientific publication.

Multi-model growth analysis with `Estimate_Growth()`

Estimate_Growth() is the main function for length-at-age modelling and offers the user a lot of flexibility which we will run through. This function applies the multi-model approach outlined in Smart et al (2016) and will give the user the option of applying three growth models. These include the von Bertalanffy growth model: L_a = L₀ + (L_∞ − L₀)(1 − e^−ka) the Gompertz model: L_a = L₀e^{log(L_∞/L₀)(1 − e^−ga)}) and the Logistic model: L_a = (L_∞ * L₀ * e^ga)/(L_∞ + L₀ * e^ga − 1) where L_a is length-at-age a, L_∞ is the asymptotic length and L₀ is the length-at-birth. Each model has its own growth coefficient (k = von Bertalanfy, g = Gompertz and g = Logistic). These growth coefficients are incomparable between models (though the Gompertz and Logistic models share the same notation) whereas the L_∞ and L₀ have the same interpretation between models.

Most applications of growth models for fish have used a t₀ rather than L₀ which represents the time-at-length-zero rather than a length-at-birth (i.e time-zero). However, t₀ does not have the same definition across multiple growth models whereas L₀ always refers to length-at-birth. This parameter is therefore used a multi-model approach. t₀ can be calculated from the VBGF parameters as: t₀ = (1/k)log(L_∞ − L₀)/L_∞)

Each of these models provide different growth forms and applying them simultaneously and comparing their fits will increase the chance that an appropriate model is applied to the data. Alternatively, the user can apply a single model or select a combination (see further down).

Using Estimate_Growth() will perform the following:

Fit the specified growth models using an nls() function, returning the parameters and summary statistics.
Calculate Akaike’s information criterion (AIC) values and calculate model weights (w).
Bootstrap the data and return 95% confidence intervals for a growth curve over the age range of the data.
Return a plot with the growth curves with confidence intervals (can be disabled to return raw results).
Return parameter correlation matrices when requested.

Input data

The data argument of Estimate_Growth() requires a data.frame of length and age data. However, this function is flexible and does not require the data to be preprocessed. The function will automatically determine the “Age” column and the “Length” column by looking for sensibly named columns. For example, the length column will accept a column named “Length”, “Len”, “STL”, “TL”, “LT” or “size”. If a column can’t be distinguished the user will be prompted to rename the necessary column. This setup means that a data set that contains additional columns (such as “Sex” or “Date”) can be passed to the function. The only issue the user should be aware of is that multiple length columns can’t be provided (i.e “TL” and “FL”).

Example applications

Growth functions can be tested using an included data set which contains length-at-age data for common blacktip sharks (Carcharhinus limbatus) from Indonesia.

library(AquaticLifeHistory)

data("growth_data")

head(growth_data) 
#>   Age    Length Sex
#> 1   0  628.7329   F
#> 2   1  895.8052   F
#> 3   2 1098.3352   F
#> 4   3 1287.5114   F
#> 5   4 1471.1238   F
#> 6   5 1584.6282   F

Length-at-age 95% confidence intervals will be produced for the growth curve through bootstrapping with 1000 iterations being default. The Estimate_growth() will return a list of parameter estimates for three candidate models: the von Bertalanffy growth function (“VB”), Gompertz growth function (“Gom”) and Logistic growth function (“Log”). The AIC results for each model will also be returned as a list element, demonstrating which growth model best fit the data. A plot will be printed with the growth curves for all included models.

Estimate_Growth(data = growth_data)

#> $VonB
#>         Parameter           SE
#> Linf 2439.5652159 50.416332563
#> k       0.1253499  0.006938586
#> L0    659.2971933 14.318722999
#> RSE    97.9461196           NA
#> 
#> $Logistic
#>         Parameter          SE
#> Linf 2163.8640279 26.46867771
#> g       0.2894532  0.01039625
#> L0    727.0885218 13.58771355
#> RSE   110.3850532          NA
#> 
#> $Gompertz
#>        Parameter           SE
#> Linf 2250.137001 32.621705588
#> g       0.207524  0.008455423
#> L0    695.603479 13.779413935
#> RSE   103.855273           NA
#> 
#> $AIC
#>   Model    AICc AIC diff Weight
#> 1    VB 3535.10     0.00      1
#> 2   Log 3605.40    70.30      0
#> 3   Gom 3569.54    34.45      0

The Estimate_Growth() function produces these outputs in three steps:

Starting parameters for the models are determined using the Ford-Walford method.
The models are fit to the data using the nls() function.
- The nls() function can be very fragile and prone to non-convergence if the data is not well suited to a particular model. Using the models argument to specify different models is a good way to initially exclude problematic growth models if a non-convergence error message occurs.
Bootstrapping is performed to determine 95% confidence intervals along the growth curve. These are included in the plot or returned to the user if plots = FALSE is used.
- The bootstrap iterations are specified using the n.bootstraps argument. The default is 1000.

Fitting specific models

One model can also be specified using the models argument:

Estimate_Growth(data = growth_data, models = "VB")

#> $VonB
#>         Parameter           SE
#> Linf 2439.5652159 50.416332563
#> k       0.1253499  0.006938586
#> L0    659.2971933 14.318722999
#> RSE    97.9461196           NA
#> 
#> $AIC
#>   Model   AICc AIC diff Weight
#> 1    VB 3535.1        0      1

Or several can be specified:

Estimate_Growth(data = growth_data, models = c("Log", "Gom"))

#> $Logistic
#>         Parameter          SE
#> Linf 2163.8640279 26.46867771
#> g       0.2894532  0.01039625
#> L0    727.0885218 13.58771355
#> RSE   110.3850532          NA
#> 
#> $Gompertz
#>        Parameter           SE
#> Linf 2250.137001 32.621705588
#> g       0.207524  0.008455423
#> L0    695.603479 13.779413935
#> RSE   103.855273           NA
#> 
#> $AIC
#>   Model    AICc AIC diff Weight
#> 1   Log 3605.40    35.85      0
#> 2   Gom 3569.54     0.00      1

Note: The three models must be specified as “VB”, “Log” and “Gom”. Otherwise there will be an error

Estimate_Growth(data = growth_data, models = "VBGF")

#> Error in Estimate_Growth(data = growth_data, models = "VBGF", n.bootstraps = 10): Models an only be 'VB', 'Log', 'Gom' or a combination of these

Plot alterations

If you are plotting one model you probably don’t want a legend included. This can be removed using the plot.legend argument. This also works when several models are plotted

Results <- Estimate_Growth(data = growth_data, models = "VB", plot.legend = FALSE)

Lastly, the y-axis label will automatically scale the unit from mm to cm based on the input data

new.dat <- growth_data
new.dat$Length <- new.dat$Length/10

Results <- Estimate_Growth(new.dat)

Returning length-at-age estimates

It is recommended that users create their own plots for publications. Therefore setting plots = FALSE will provide these estimates as an additional list object rather than printing a plot.

results <- Estimate_Growth(data = growth_data, plots = FALSE)

Length_at_age_estimates <- results$Estimates

head(Length_at_age_estimates)

#>             Model Age      AVG      low      upp
#> 1 Von Bertalanffy 0.0 659.2972 637.5146 680.1881
#> 2 Von Bertalanffy 0.1 681.4736 660.2606 702.2148
#> 3 Von Bertalanffy 0.2 703.3737 682.7219 724.0287
#> 4 Von Bertalanffy 0.3 725.0010 704.9021 745.5965
#> 5 Von Bertalanffy 0.4 746.3589 726.8047 766.8886
#> 6 Von Bertalanffy 0.5 767.4507 748.4331 787.9083

Model averaged results with `Calculate_MMI`

Multi-model inference (MMI) can be useful when no particular candidate model provides a better fit than the others (ΔAIC < 2). This involves averaging all models across the growth curve based on their AIC weights (w). It will return model averaged values of L_∞ and L₀ with averaged standard errors. No model averaged growth completion parameters (k for VBGF, g for Gompertz or g for Logistic) are returned as these parameters are not comparable across models.

Calculate_MMI takes the outputs of Estimate_Growth with plots = FALSE (so that length-at-age estimates are available) and will calculate MMI parameters and standard errors as well as model averaged length-at-age estimates.

results <- Estimate_Growth(data = growth_data,  plots = FALSE)
Calculate_MMI(results)

#> $`MMI parametrs`
#>   Parameter       AVG       SE
#> 1      Linf 2439.5652 50.41633
#> 2        L0  659.2972 14.31872
#> 
#> $`MMI estimates`
#>    Age       AVG
#> 1    0  659.2972
#> 2    1  870.0338
#> 3    2 1056.0610
#> 4    3 1220.2898
#> 5    4 1365.2883
#> 6    5 1493.3221
#> 7    6 1606.3897
#> 8    7 1706.2545
#> 9    8 1794.4719
#> 10   9 1872.4140
#> 11  10 1941.2914
#> 12  11 2002.1721
#> 13  12 2055.9981
#> 14  13 2103.6006
#> 15  14 2145.7128
#> 16  15 2182.9814
#> 17  16 2215.9772
#> 18  17 2245.2035

Be aware that bootstrapping cannot be applied for MMI so no length-at-age errors are available. Also multi-model theory dictates that model averaged errors will be larger than the cumulative errors of candidate models. So don’t be alarmed if you get large parameter standard errors.

Sex specific growth curves

Separating the sexes is common and is achieved by sub setting data and running the function multiple times. They can then be combined and plotted afterwards. This can be done for one model or several. Here is an example using the ggplot2 package.

# Create data.frames of separate sexes
Females <- dplyr::filter(growth_data, Sex == "F")
Males <- dplyr::filter(growth_data, Sex == "M")

# Estimate growth
Female_ests <- Estimate_Growth(Females,n.bootstraps = 1000, plots = FALSE)
Male_ests <- Estimate_Growth(Males, n.bootstraps = 1000,plots = FALSE)

# Combine data sets with a new variable designating sex
Female_LAA <- Female_ests$Estimates
Female_LAA$Sex <- "F"

Male_LAA <- Male_ests$Estimates
Male_LAA$Sex <- "M"

combined_data <- rbind(Male_LAA, Female_LAA)

library(ggplot2)

ggplot(combined_data, aes(x = Age, y = AVG, fill = Model, col = Model)) +
  facet_wrap(~Sex, ncol = 1, scales = "free")+
  geom_point(data = Males, aes(x = Age, y = Length, fill = NULL, col = NULL), alpha = .3) +
  geom_point(data = Females, aes(x = Age, y = Length, fill = NULL, col = NULL), alpha = .3) +
  geom_ribbon(aes(ymin = low, ymax = upp, col = NA), alpha = 0.2)+
  geom_line(size = 1)+
  scale_y_continuous(name = "Length (mm)", limits = c(0,2500), expand = c(0,0))+
  scale_x_continuous(name = "Age (years)", limits = c(0,18), expand = c(0,0))+
  theme_bw()

Combining two and three parameter models

It is common practice for fish species to fix L₀ as this parameter has a direct relation to length-at-birth (often zero for fish). This is done via a single argument Birth.Len. If this is unspecified then three parameter models are used. However, if Birth.Len is specified then two parameters are used with that value used to fix the L₀ parameter.

Estimate_Growth(growth_data, Birth.Len = 600)

#> $VonB
#>         Parameter           SE
#> Linf 2350.0363632 37.543989270
#> k       0.1436146  0.005763834
#> RSE   100.4842123           NA
#> 
#> $Logistic
#>         Parameter           SE
#> Linf 2050.9793958 19.509968061
#> g       0.3807714  0.008422721
#> RSE   124.9385671           NA
#> 
#> $Gompertz
#>         Parameter           SE
#> Linf 2148.7052970 23.646000409
#> g       0.2542661  0.006791104
#> RSE   111.5420183           NA
#> 
#> $AIC
#>   Model    AICc AIC diff Weight
#> 1    VB 3549.09     0.00      1
#> 2   Log 3677.17   128.08      0
#> 3   Gom 3610.48    61.39      0

These can be plotted alongside there three parameter versions as well. Here is an example for the VBGF

# Fit models
two_pars <- Estimate_Growth(growth_data, models = "VB", Birth.Len = 600, plots = FALSE)
three_pars <- Estimate_Growth(growth_data, models = "VB", plots = FALSE)

# Change Model name to represent how many parameters they have
two_pars_Ests <- two_pars$Estimates
two_pars_Ests$Model <- "2 parameter VBGF"

three_pars_Ests <- three_pars$Estimates
three_pars_Ests$Model <- "3 parameter VBGF"

combined_data <- rbind(two_pars_Ests, three_pars_Ests)

ggplot(combined_data, aes(x = Age, y = AVG, fill = Model, col = Model)) +
  geom_point(data = growth_data, aes(x = Age, y = Length, fill = NULL, col = NULL), alpha = .3) +
  geom_ribbon(aes(ymin = low, ymax = upp, col = NA), alpha = 0.2)+
  geom_line(size = 1)+
  scale_y_continuous(name = "Length (mm)", limits = c(0,2500), expand = c(0,0))+
  scale_x_continuous(name = "Age (years)", limits = c(0,18), expand = c(0,0))+
  theme_bw() +
  theme(legend.position = c(0.8, 0.2))

#> Warning: A numeric `legend.position` argument in `theme()` was deprecated in ggplot2
#> 3.5.0.
#> ℹ Please use the `legend.position.inside` argument of `theme()` instead.
#> This warning is displayed once every 8 hours.
#> Call `lifecycle::last_lifecycle_warnings()` to see where this warning was
#> generated.

A two parameter model is demonstrated here for sharks as this is the data used in these examples. However, fixing growth parameters for sharks is less appropriate than techniques such as back-calculation. However, for fish species, length-at-birth is close to zero due to their larval life stage. Therefore, length-at-birth is commonly fixed at zero to represent this.

Correlation matrices

Each of the growth models fit in these analyses have a multivariate normal distribution. This means that each parameter has a normal distribution but are correlated to one another. Therefore, if you would like to use these parameters in simulation analyses, a correlation matrix is needed to include parameter values in the simulations. This is not an analysis that is included in this package. However, the ability to return the correlation matrix is included in the Estimate_Growth() function by using correlation.matrix = TRUE.

Estimate_Growth(growth_data, correlation.matrix = TRUE)

#> $VonB
#>            Linf          k         L0
#> Linf  1.0000000 -0.9617305  0.4476484
#> k    -0.9617305  1.0000000 -0.5986417
#> L0    0.4476484 -0.5986417  1.0000000
#> 
#> $Logistic
#>            Linf          g         L0
#> Linf  1.0000000 -0.8190773  0.4284394
#> g    -0.8190773  1.0000000 -0.7632670
#> L0    0.4284394 -0.7632670  1.0000000
#> 
#> $Gompertz
#>            Linf          g         L0
#> Linf  1.0000000 -0.8981026  0.4488018
#> g    -0.8981026  1.0000000 -0.6991582
#> L0    0.4488018 -0.6991582  1.0000000

Full details on these approaches can be found in: Smart, J. J., Chin, A. , Tobin, A. J. and Simpfendorfer, C. A. (2016) Multimodel approaches in shark and ray growth studies: strengths, weaknesses and the future. Fish and Fisheries, 17: 955-971.https://onlinelibrary.wiley.com/doi/abs/10.1111/faf.12154 which should be cited if this package is used in a publication.

Growth estimation example using the AquaticLifeHistory package